Política de armazenamento de dados em nuvens federadas para dados biológicos

Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Exatas, Departamento de Ciência da Computação, 2014.A computação em nuvem tem possibilitado a integração de diversos provedores para a execução de tarefas de forma mais rápida em comparação a utilização dos modelos anteriores,e uma visão ao usuário de que os recursos de armazenamento e processamento são ilimitados. A Bioinformática, que lida com grande volume de informações, pode utilizar-se da infraestrutura de computação em nuvem para disponibilizar suas ferramentas,para serem utilizadas nos fluxos de trabalhos, chamados workflows. Além disso, as instituições podem associar-se a outras instituições para formar uma federação de nuvens computacionais, proporcionando maior flexibilidade na escolha de provedores de serviço.Neste contexto, o desempenho da execução de workflows de Bioinformática é fortemente afetado pelo armazenamento e recuperação de dados, devido ao grande volume de informações das sequências genômicas. Desta forma, a escolha da melhor nuvem para estas operações é fundamental para a eficiência da execução do workflow. O presente trabalho tem por objetivo propor uma política de armazenamento de dados genômicos para nuvens computacionais federadas buscando, através da definição de alguns critérios de seleção e estratégias, diminuir o tempo de transferência dos dados e assim contribuir para a diminuição do tempo total de execução do workflow. Foi realizado um estudo de caso, com dados reais, utilizando a plataforma BioNimbuZ[1], que é uma arquitetura para execução de workflows de bioinformática no ambiente de nuvens federadas. Com os resultados obtidos, foi possível determinar o peso de cada critério da política de armazenamento e realizar as análises em relação a política originalmente criada para a arquitetura. Desta forma, a política de armazenamento proposta apresentou ganhos quanto a eficiência, principalmente em nuvens com grande poder computacional. ________________________________________________________________________________ ABSTRACTCloud computing has enabled the integration of providers to perform tasks faster, and has allowed users to view the processing and storage resources as unlimited. Bio informatics, which handles large volume of information produced by high-throughput sequencing machines, may use the infrastructure of cloud computing to provide tools to be used in workflows. In addition, institutions may join other institutions to form a federation of computing clouds, providing greater flexibility in the choice of service providers. In this context, the performance of the implementation of a workflow is strongly affected by the storage and retrieval of data, due to the large amount of information from genomic sequences. Thus, choosing the best cloud for these operations is critical to the efficiency of the workflow. This work aims to propose a policy for the storage of genomic data for federated cloud computing seeking, by defining some selection criteria and strategies, toreduce the time of data transfer and thus contribute to the reduction of total execution time of the workflow. A case study was carried out with real data, using BioNimbuZ platform, which is an architecture for the implementation of bioinformatics workflows infederated cloud environments. With the results obtained, it was possible to determine the weight of each storage policy criteria and perform the analysis regarding the policy originally created for the architecture. Thus, the storage policy proposal presented efficiency gains, especially in clouds with great computing power

Desenvolvimento de metodologia para o uso de geotecnologias de baixo custo aplicada à análise da situação arbórea no município de Sinop, MT

The geospatial technologies are becoming a very helpful tool for decision making. The use of Geographic Information Systems can optimize the mapping of environmental changes detection. In the urban management, the use of technologies for geospatial analysis is very important for both implantation planning and change strategy of the urban space. In the arborization scope, its very important to perform a previous study of the area taking as account the local geographical and structural aspects to facilitate the right decision in the planning about the types and distribution of the trees. The urban vegetation coverage is an aspect that has direct influence in the population. Therefore, the aim of this paper is to present a methodology developed to analyze the tree distribution in urban area of Sinop-MT and its environmental, social and urban impacts. For this, it were used geo-technologies of low cost as a low price GPS mobile, free satellite image and freeware SIG. For this study it was chosen tree major areas to collect the data which are: commercial area, consolidated and non-consolidated residential area. In this paper we present the case study of Ipanema Residential, which was chosen as one of the non-consolidated residential area. We intend to develop an urban arborization planning for the city of Sinop, based on the analysis performed in the study areas.Pages: 901-90

RESISTÊNCIA À DETERIORAÇÃO DE MADEIRAS AMAZÔNICAS TRATADAS POR IMERSÃO SIMPLES EM ÓLEO QUEIMADO

Este estudo teve como objetivo avaliar a resistência à deterioração de madeiras amazônicas tratadas por imersão simples em óleo queimado. Para tanto, amostras de Trattinnickia rhoifolia (amescla) e Qualea albiflora (cambará), com dimensões de 2 x 2 x 20 cm, foram submetidas aos tratamentos preservativos sem pressão em três intervalos de tempo 1, 3 e 5 h. A eficiência dos tratamentos utilizando óleo queimado foi determinada pela perda de massa e o índice de deterioração das amostras tratadas e não tratadas de amescla e cambará submetidas aos ensaios de deterioração de campo, durante um período de 10 meses. O tempo de imersão em óleo queimado não influenciou na taxa de retenção do preservativo nas madeiras de amescla e cambará. Dentre as madeiras ensaiadas, o cambará apresentou a melhor tratabilidade e consequente resistência à deterioração em comparação a amescla. O emprego do óleo queimado foi eficiente, de modo a proporcionar uma maior resistência à deterioração das madeiras de amescla e cambará, destacando-se o tempo de imersão de 5 h. Palavras-chave: preservação da madeira, madeira tropical, ensaios de campo, perda de massa. RESISTANCE TO DETERIORATION OF AMAZONIAN WOOD TREATED BY SHORT-TERM SOAK IN USED ENGINE OIL ABSTRACT This study aimed to evaluate the resistance to deterioration of Amazonian wood treated by short-term soak in used engine oil. For this purpose samples of Trattinnickia rhoifolia (amescla) and Qualea albiflora (cambará), with dimensions of 2 x 2 x 20 cm, were submitted to the preservative treatments at three time slots 1, 3 and 5 hours. The efficiency of the used engine oil was determined by comparing the mass loss and decay index of amescla and cambará wood treated and untreated submitted in field tests for 10 months. The soak time in used oil engine did not influence the preservative retention rate in the amescla and cambará wood. Among the tested wood, cambará wood presented the best treatability and consequent resistance to deterioration compared to amescla wood. The use of used oil engine was efficient, providing a greater resistance to deterioration of amescla and cambará wood, especially when considering the soak time of 5 h. Keywords: wood preservation, tropical wood, field tests, mass loss. DOI: http://dx.doi.org/10.14583/2318-7670.v02n01a0

Cellular and molecular immune profiles in renal transplant recipients after conversion from Tacrolimus to Sirolimus

Tacrolimus and Sirolimus are commonly used maintenance immunesuppressants in kidney transplantation. Since their effects on immune cells and allograft molecular profiles have not been elucidated, we characterized the effects of Tacrolimus to Sirolimus conversion on frequency and function of T cells, and on graft molecular profiles. Samples from renal transplant patients in a randomized trial of 18 patients with late Sirolimus conversion and 12 on Tacrolimus maintenance were utilized. Peripheral blood was collected at 0, 6, 12 and 24-months post-randomization with T cell subpopulations analyzed by flow cytometry and T cell alloreactivity tested by IFN-γ ELISPOT. Graft biopsy samples obtained 24-months post-randomization were used for gene expression analysis. Sirolimus conversion led to an increase in CD4+25+++Foxp3+ regulatory T cells. While Tacrolimus-maintained patients showed a decrease in indirect alloreactivity over time post-transplant, Sirolimus conversion increased indirect alloreactive T cell frequencies compared to Tacrolimus-maintained patients. No histological differences were found in graft biopsies, but molecular profiles showed activation of the antigen presentation, IL-12 signaling, oxidative stress, macrophage-derived production pathways, and increased inflammatory and immune response in Sirolimus-converted patients. Thus, chronic immune alterations are induced after Sirolimus conversion. Despite the molecular profile being favorable to calcineurin inhibitor-based regimen, there was no impact in renal function over 30 months of follow-up